
I need following tasks to be done. Python and Machine Learning.
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Description
Experience Level: Entry
I need a freelancer who is expert in writing original article by extracting data from the following portals.
1.The NCBI GEO database (http://www.ncbi.nlm.nih.gov/geo)
2. The original SRA file should be downloaded and processed
using SRA-Toolkit (https://github.com/ncbi/sra-tools/wiki/01.-Down
loading-SRA-Toolkit) and Salmon (https://salmon.readthedocs.io/en
/latest/salmon.html) to generate FASTQ and gene count files for
further analysis.
3.Additionally, the GSE235995 expression profiling
dataset,should be sequenced using the Illumina NovaSeq 6000 (GPL24676) for
Homo sapiens, was acquired from the GEO database.
4. This dataset which will be divided in to 2 groups should include
five calcific aortic valve disease samples and four normal
control samples.
5. Data standardization and log₂ (data+1) transformation
should be performed using the Python and R package ‘DESeq2’ [10] (https://biocon
ductor.org/packages/release/bioc/html/DESeq2.html), and this dataset will be used for validation.
1.The NCBI GEO database (http://www.ncbi.nlm.nih.gov/geo)
2. The original SRA file should be downloaded and processed
using SRA-Toolkit (https://github.com/ncbi/sra-tools/wiki/01.-Down
loading-SRA-Toolkit) and Salmon (https://salmon.readthedocs.io/en
/latest/salmon.html) to generate FASTQ and gene count files for
further analysis.
3.Additionally, the GSE235995 expression profiling
dataset,should be sequenced using the Illumina NovaSeq 6000 (GPL24676) for
Homo sapiens, was acquired from the GEO database.
4. This dataset which will be divided in to 2 groups should include
five calcific aortic valve disease samples and four normal
control samples.
5. Data standardization and log₂ (data+1) transformation
should be performed using the Python and R package ‘DESeq2’ [10] (https://biocon
ductor.org/packages/release/bioc/html/DESeq2.html), and this dataset will be used for validation.

Sanjiv N.
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30 Apr 2025
United Kingdom
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